M-FC Agar

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

M-FC Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Tryptose......................................................................................10.0g
Inositol........................................................................................10.0g
Proteose peptone...........................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
Bile salts mixture..........................................................................1.5g
Aniline Blue..................................................................................0.1g
Selective supplement solution .................................................10.0mL
Rosolic acid solution................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Rosolic Acid Solution:
Composition per 10.0mL:
Rosolic acid ..................................................................................0.1g
Preparation of Rosolic Acid Solution:  Add rosoloic acid to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Selective Supplement Solution:
Composition per 10.0mL:
Carbenicillin................................................................................0.05g
Preparation of Selective Supplement Solution:  Add carbenicil-
lin to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add selective supplement solution.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the rapid enumeration of Klebsiella using the membrane fil-
ter technique.