M-FC HiVeg Agar Base with Rosalic Acid

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

M-FC HiVeg Agar Base with Rosalic Acid

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Lactose........................................................................................12.5g
Plant hydrolysate No. 1...............................................................10.0g
Plant peptone No. 3.......................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................3.0g
Synthetic detergent No. I ..............................................................1.5g
Aniline Blue..................................................................................0.1g
Rosolic acid solution................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium, without rosolic acid, is available as a premixed
powder from HiMedia.
Rosolic Acid Solution:
Composition per 100.0mL:
Rosolic acid...................................................................................1.0g
Preparation of Rosolic Acid Solution: Add rosolic acid to 0.2N
NaOH and bring volume to 100.0L. Mix thoroughly.
Preparation of Medium: Add 10.0mL rosolic acid solution to
950.0mL of distilled/deionized water. Mix thoroughly. Add other com-
ponents and bring volume to 1.0L with distilled/deionized water. Mix
thoroughly. Gently heat and bring to boiling with frequent mixing. Do
not autoclave. Pour into sterile Petri dishes or leave in tubes.
Use: For the detection and enumeration of fecal coliforms using the
membrane filter technique.