Methylpyridine Medium

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methylpyridine Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1002.0mL:

K 2 HPO 4 .......................................................................................0.61g
KH 2 PO 4 .......................................................................................0.39g
KCl..............................................................................................0.25g
Yeast extract..................................................................................0.1g
Wolfe’s mineral solution..........................................................10.0mL
2-Methylpyridine.......................................................................1.0mL
Wolfe’s Mineral Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoCl 2 ·6H 2 O..................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
CaCl 2 ·2H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
CuSO 4 ·5H 2 O...............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O..........................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components one at a time. Add distilled/deion-
ized water to 1.0L. Adjust pH to 6.8.
Preparation of Medium: Add components, except 2-methylpyri-
dine, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room
temperature. In a fume hood, aseptically add 1.0mL of 2-methylpyri-
dine. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use polyurethane foam closures to eliminate odors caused by volatil-
ization of 2-methylpyridine.
Use: For the cultivation of Arthrobacter species.