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Methylosarcina quisquillarum/ Methylosarcina fibrata Medium (DSMZ Medium 921)
[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]
Methylosarcina quisquillarum/
Methylosarcina fibrata Medium
(DSMZ Medium 921)
Phosphate buffer......................................................................10.0mL
Solution 3...................................................................................1.0mL
Trace elements...........................................................................1.0mL
Solution 2...................................................................................0.1mL
pH 7.0 ± 0.2 at 25°C
Solution 1 (10X NMS Salts):
Composition per liter:
KNO 3 ..........................................................................................10.0g
MgSO 4 ·6H 2 O..............................................................................10.0g
CaCl 2 ·2H 2 O ..................................................................................2.0g
Preparation of Solution 1 (10X NMS Salts): Add components
to 700.0mL distilled/deionized water. Mix thoroughly. Bring volume to
1.0L with distilled/deionized water. Mix thoroughly.
Solution 2 (Fe EDTA):
Composition per liter:
Fe EDTA.......................................................................................3.8g
Preparation of Solution 2 (Fe EDTA): Add Fe EDTA to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Solution 3 (Sodium Molybdate):
Composition per liter:
Na 2 MoO 4 ·4H 2 O ..........................................................................0.26g
Preparation of Solution 3 (Sodium Molybdate): Add
Na 2 MoO 4 ·4H 2 O to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly.
Trace Elements:
Composition per 100.0mL:
CuSO 4 ·5H 2 O..........................................................................100.0mg
FeSO 4 ·7H 2 O.............................................................................50.0mg
ZnSO 4 ·7H 2 O............................................................................40.0mg
EDTA disodium salt.................................................................25.0mg
CoCl 2 ·6H 2 O ...............................................................................5.0mg
MnCl 2 ·4H 2 O...............................................................................2.0mg
H 3 BO 3 ........................................................................................1.5mg
NiCl 2 ·6H 2 O................................................................................1.0mg
Preparation of Trace Elements: Add components to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly.
Phosphate Buffer:
Composition per liter:
Na 2 HPO 4 ·2H 2 O...........................................................................71.6g
KH 2 PO 4 .......................................................................................26.0g
Preparation of Phosphate Buffer: Add components to 800.0mL
distilled/deionized water. Mix thoroughly. Adjust pH to 6.8. Bring vol-
ume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 55°C.
Preparation of Medium: Add 100.0mL solution 1 to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Add 1.0mL
of solution 3, 1.0mL of the trace elements, and 0.1mL of solution 2.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 55°C. Asepti-
cally add 10.0mL phosphate buffer. Mix thoroughly. Aseptically dis-
tribute to sterile tubes or bottles.
Use: For the cultivation of Methylosarcina fibrata and Methylosarcina
quisquiliarum.
Methylosarcina fibrata Medium
(DSMZ Medium 921)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per 1012.1mL:
Solution 1...............................................................................100.0mLPhosphate buffer......................................................................10.0mL
Solution 3...................................................................................1.0mL
Trace elements...........................................................................1.0mL
Solution 2...................................................................................0.1mL
pH 7.0 ± 0.2 at 25°C
Solution 1 (10X NMS Salts):
Composition per liter:
KNO 3 ..........................................................................................10.0g
MgSO 4 ·6H 2 O..............................................................................10.0g
CaCl 2 ·2H 2 O ..................................................................................2.0g
Preparation of Solution 1 (10X NMS Salts): Add components
to 700.0mL distilled/deionized water. Mix thoroughly. Bring volume to
1.0L with distilled/deionized water. Mix thoroughly.
Solution 2 (Fe EDTA):
Composition per liter:
Fe EDTA.......................................................................................3.8g
Preparation of Solution 2 (Fe EDTA): Add Fe EDTA to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Solution 3 (Sodium Molybdate):
Composition per liter:
Na 2 MoO 4 ·4H 2 O ..........................................................................0.26g
Preparation of Solution 3 (Sodium Molybdate): Add
Na 2 MoO 4 ·4H 2 O to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly.
Trace Elements:
Composition per 100.0mL:
CuSO 4 ·5H 2 O..........................................................................100.0mg
FeSO 4 ·7H 2 O.............................................................................50.0mg
ZnSO 4 ·7H 2 O............................................................................40.0mg
EDTA disodium salt.................................................................25.0mg
CoCl 2 ·6H 2 O ...............................................................................5.0mg
MnCl 2 ·4H 2 O...............................................................................2.0mg
H 3 BO 3 ........................................................................................1.5mg
NiCl 2 ·6H 2 O................................................................................1.0mg
Preparation of Trace Elements: Add components to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly.
Phosphate Buffer:
Composition per liter:
Na 2 HPO 4 ·2H 2 O...........................................................................71.6g
KH 2 PO 4 .......................................................................................26.0g
Preparation of Phosphate Buffer: Add components to 800.0mL
distilled/deionized water. Mix thoroughly. Adjust pH to 6.8. Bring vol-
ume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 55°C.
Preparation of Medium: Add 100.0mL solution 1 to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Add 1.0mL
of solution 3, 1.0mL of the trace elements, and 0.1mL of solution 2.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 55°C. Asepti-
cally add 10.0mL phosphate buffer. Mix thoroughly. Aseptically dis-
tribute to sterile tubes or bottles.
Use: For the cultivation of Methylosarcina fibrata and Methylosarcina
quisquiliarum.
