Methylocapsa acidophila Medium (DSMZ Medium 922)

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methylocapsa acidophila Medium
(DSMZ Medium 922)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

KH 2 PO 4 ..................................................................................100.0mg
MgSO 4 ·7H 2 O...........................................................................50.0mg
CaCl 2 ·2H 2 O..............................................................................10.0mg
Trace elements...........................................................................1.0mL
pH 4.5-5.8 at 25°C
Trace Elements:
Composition per liter:
EDTA............................................................................................5.0g
FeSO 4 ·7H 2 O..................................................................................2.0g
CoCl 2 ·6H 2 O ..................................................................................0.2g
CuCl 2 ·5H 2 O ..................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
Na 2 MoO 4 .....................................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
Preparation of Trace Elements: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. The final pH
should be 4.5–5.8. The medium is fairly weakly buffered so the pH
should be checked before and after autoclaving. Incubate under atmo-
sphere of 10–30% methane.
Use: For the cultivation of Methylocapsa acidiphila.