Methylobacterium thiocyanatum Medium (DSMZ Medium 805)

[所属分类:培养基配方] [发布时间:2021-5-27] [发布人:网站管理员2] [阅读次数:] [返回]

Methylobacterium thiocyanatum Medium
(DSMZ Medium 805)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na 2 HPO 4 ·2H 2 O.............................................................................7.9g
Glucose .........................................................................................4.5g
K 2 HPO 4 .........................................................................................1.5g
KSCN..........................................................................................0.25g
MgSO 4 ·7H 2 O................................................................................0.1g
Iron sulfate solution...................................................................1.0mL
pH 7.1 ± 0.1 at 25°C
Iron Sulfate Solution:
Composition per 10.0mL:
FeSO 4 ·7H 2 O..................................................................................0.2g
Preparation of Iron Sulfate Solution:  Add FeSO 4 ·7H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring to 1.0L. Mix thoroughly. Distribute into tubes or
flasks. Autoclave for 10 min at 10 psi pressure–115°C.
Use: For the cultivation of Methylobacterium thiocyanatum.