Methanogenium bourgense Medium

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanogenium bourgense Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium formate ............................................................................5.0g
Sodium acetate..............................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
Trypticase™..................................................................................1.0g
Yeast extract..................................................................................1.0g
L -Cysteine·HCl .............................................................................0.5g
K 2 HPO 4 ·3H 2 O ..............................................................................0.4g
MgCl 2 ·6H 2 O .................................................................................0.1g
Resazurin ...................................................................................1.0mg
Na 2 CO 3  solution.......................................................................10.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
pH 6.7 ± 0.2 at 25°C
Na 2 CO 3 Solution:
Composition per 10.0mL:
Na 2 CO 3 .........................................................................................1.5g
Preparation of Na 2 CO 3 Solution:  Add Na 2 CO 3 to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Sparge with
100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.2g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Prepare and dispense medium under 80%
H 2 + 20% CO 2 . Add components, except Na 2 CO 3 solution and
Na 2 S·9H 2 O solution, to distilled/deionized water and bring volume to
980.0mL. Mix thoroughly. Adjust pH to 67. Anaerobically distribute
into tubes or flasks fitted with butyl rubber stoppers. Autoclave for 15
min at 15 psi pressure–121°C. Anaerobically add 10.0mL of sterile
Na 2 CO 3 solution and 10.0mL of sterile Na 2 S·9H 2 O solution to each li-
ter of medium or, using a syringe, inject the appropriate amount of ster-
ile Na 2 CO 3 solution and sterile Na 2 S·9H 2 O solution into individual
tubes containing medium.
Use: For the cultivation and maintenance of Methanogenium bour-
gense.