Methanogenium bourgense Medium (DSMZ Medium 322)

[所属分类:培养基配方] [发布时间:2021-5-25] [发布人:网站管理员2] [阅读次数:] [返回]

Methanogenium bourgense Medium
(DSMZ Medium 322)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Na-formate....................................................................................5.0g
Na 2 CO 3 .........................................................................................1.5g
Na-acetate.....................................................................................1.0g
Trypticase™ peptone....................................................................1.0g
Yeast extract..................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
K 2 HPO 4 ·3H 2 O ..............................................................................0.4g
MgCl 2 ·6H 2 O .................................................................................0.1g
Resazurin ...................................................................................1.0mg
Cysteine solution .....................................................................10.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
pH 6.0–7.0 at 25°C
Cysteine Solution:
Composition per 10.0mL:
L -Cysteine·HCl·H 2 O.....................................................................0.5g
Preparation of Cysteine Solution:  Add  L -cysteine·HCl·H 2 O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Na 2 S·9H 2 O Solution :
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.2g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave under 100% N 2 for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Preparation of Medium: Prepare and dispense medium under an
oxygen-free 80% H 2 + 20% CO 2  gas mixture. Add components, except
cysteine solution and Na 2 S·9H 2 O solution, to distilled/deionized water
and bring volume to 980.0mL. Mix thoroughly. Sparge with 80% H 2 +
20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C while sparging with 80% H 2 + 20% CO 2 . Aseptically and anaer-
obically add 10.0mL of sterile cysteine solution and 10.0mL of sterile
Na 2 S·9H 2 O solution. Mix thoroughly. Aseptically and anaerobically
distribute into sterile tubes or flasks.
Use:  For the cultivation of Methanoculleus bourgensis=Methanoge-
nium bourgense.