A 2 E 6 Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

A 2 E 6 Medium
Composition per liter:
NaCl..........................................................................................23.48g
MgCl 2 ·6H 2 O..............................................................................10.63g
Na 2 SO 4 ........................................................................................3.92g
Glucose .........................................................................................3.0g
Tris buffer .....................................................................................3.0g
Glutamic acid................................................................................1.5g
CaCl 2 , anhydrous........................................................................1.11g
KCl..............................................................................................0.66g
NaHCO 3 ......................................................................................0.19g
Sodium glycerophosphate...........................................................0.15g
KBr................................................................................................0.1g
(NH 4 ) 2 SO 4 ...................................................................................0.05g
SrCl 2 ·6H 2 O..................................................................................0.04g
H 3 BO 3 .........................................................................................0.03g
FeCl 3 ·6H 2 O.................................................................................0.01g
K 2 HPO 4 .......................................................................................0.01g
Metal mixture.............................................................................3.0mL
Vitamin solution.........................................................................1.0mL
pH 6.4–6.6 at 25°C
Metal Mixture:
Composition per 100.0mL:
EDTA............................................................................................1.0g
H 3 BO 3 ...........................................................................................1.0g
MnCl 2 ·4H 2 O................................................................................0.15g
FeCl 3 ·6H 2 O.................................................................................0.05g
ZnCl 2 ...........................................................................................0.01g
CoCl 2 ·6H 2 O ..............................................................................0.005g
Preparation of Metal Mixture: Add components to distilled/de-
ionized water and bring volume to 100.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Thiamine.......................................................................................1.0g
Biotin........................................................................................0.003g
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 999.0mL. Mix
thoroughly. Adjust pH to 6.4–6.6. Autoclave for 15 min at 15 psi pres-
sure–121°C. Aseptically add 1.0mL of sterile vitamin solution. Mix
thoroughly. Aseptically distribute into sterile screw-capped tubes or
flasks.
Use: For the cultivation of Crypthecodinium cohnii.