AE Medium (4a/3e) (Gluconobacter Medium) (LMG Medium 269)

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

AE Medium (4a/3e)
(Gluconobacter Medium)
(LMG Medium 269)
Composition per liter:
Agar ............................................................................................15.0g
Glucose .......................................................................................10.0g
Peptone..........................................................................................3.0g
Yeast extract..................................................................................2.0g
Acetic acid, glacial...................................................................40.0mL
Ethanol, 96%............................................................................30.0mL
Preparation of Medium: Add components, except acetic acid and
ethanol, to 930.0mL distilled/deionized water. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 40.0mL filter sterilized ace-
tic acid and 30.0mL filter sterilized 96% ethanol. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation of Gluconacetobacter entanii and other Glu-
conacetobacter spp.