M-Enterococcus Agar Base, Modified

[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]

M-Enterococcus Agar Base, Modified

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Yeast extract................................................................................30.0g
Pancreatic digest of gelatin.........................................................10.0g
Agar............................................................................................15.0g
NaCl............................................................................................15.0g
Esculin ..........................................................................................1.0g
Nalidixic acid..............................................................................0.25g
NaN 3 ...........................................................................................0.15g
Cycloheximide............................................................................0.05g
Selective supplement solution .................................................15.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Caution:  Sodium azide has a tendency to form explosive metal azides
with plumbing materials. It is advisable to use enough water to flush
off the disposables.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Selective Supplement Solution:
Composition per 20.0mL:
2,3,5-Triphenyl tetrazolium chloride............................................0.2g
Preparation of Selective Supplement Solution:  Add 2,3,5-tri-
phenyl tetrazolium chloride to distilled/deionized water and bring vol-
ume to 20.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
985.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add selective supplement solution.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the recovery of enterococci in water samples using the mem-
brane filter technique.