M-Enterococcus Agar Base with Polysorbate 80 and Sodium Carbonate

[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]

M-Enterococcus Agar Base
with Polysorbate 80 and Sodium Carbonate

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................10.0g
Casein enzymic hydrolysate .......................................................15.0g
Papaic digest of soybean meal......................................................5.0g
Yeast extract..................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Glucose .........................................................................................2.0g
NaN 3 ..............................................................................................0.4g
Triphenyl tetrazolium chloride......................................................0.1g
Sodium carbonate solution.........................................................2.0mL
Polysorbate 80............................................................................0.5mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Caution:  Sodium azide has a tendency to form explosive metal azides
with plumbing materials. It is advisable to use enough water to flush
off the disposables.
Sodium Carbonate Solution:
Composition per 10.0mL:
Na 2 CO 3 .........................................................................................1.0g
Preparation of Sodium Carbonate Solution:  Add Na 2 CO 3 to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except polysorbate 80
and sodium carbonate solution, to distilled/deionized water and bring
volume to 997.5mL. Mix thoroughly. Gently heat to dissolve compo-
nents. Do not autoclave. Cool to 50°C. Add polysorbate 80 and sodium
carbonate solution. Mix thoroughly. Pour into Petri dishes or aseptical-
ly distribute into sterile tubes.
Use: For the selective isolation and enumeration of enterococci from
water, sewage, food, or other materials.