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Membrane Lactose Glucuronide Agar (MLGA)

[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]
Membrane Lactose Glucuronide Agar
(MLGA)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone........................................................................................40.0g
Lactose........................................................................................30.0g
Agar ............................................................................................10.0g
Yeast extract..................................................................................6.0g
Sodium lauryl sulfate....................................................................1.0g
Sodium pyruvate...........................................................................0.5g
5-Bromo-4-chloro-3-indoxyl-
β- D -glucuronic acid................................................................0.2g
Phenol Red....................................................................................0.2g
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available from Oxoid Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri
dishes.
Use:  For the direct enumeration of E. coli and coliforms in foods by
the membrane filtration method. The chromogenic substrate 5-bromo-
4-chloro-3-indoxyl-β- D -glucuronic acid (BCIG) is cleaved by the en-
zyme β-glucuronidase and produces a blue chromophore that builds up
within the bacterial cells. In addition, the incorporation of Phenol Red
detects lactose fermentation and results in yellow colonies when acid
is produced. Since coliform colonies are lactose positive, they will ap-
pear yellow on this medium and as E. coli colonies are both lactose and
β-glucuronidase positive, they will appear green.
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