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Membrane Clostridium perfringens Medium (m-CP Medium)

[所属分类:培养基配方] [发布时间:2021-5-21] [发布人:网站管理员2] [阅读次数:] [返回]
Membrane Clostridium perfringens Medium
(m-CP Medium)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1040.0mL:

Tryptose......................................................................................30.0g
Yeast extract................................................................................20.0g
Agar............................................................................................15.0g
Sucrose..........................................................................................5.0g
L -Cysteine·HCl·H 2 O.....................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
Bromcresol Purple......................................................................0.04g
Phenolphthalein solution .........................................................20.0mL
Indoxyl-β- D -glucoside solution.................................................8.0mL
Selective supplement solution...................................................8.0mL
Ferric chloride solution..............................................................4.0mL
pH 7.6 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Selective Supplement Solution:
Composition per 8.0mL:
D -Cycloserine................................................................................0.8g
Polymyxin B sulfate ................................................................50.0mg
Preparation of Selective Supplement Solution:  Add compo-
nents to distilled/deionized water and bring volume to 8.0mL. Mix
thoroughly. Filter sterilize.
Indoxyl- β - D -glucoside Solution:
Composition per 10.0mL:
Indoxyl-β- D -glucoside.............................................................75.0mg
Preparation of Indoxyl- β - D -glucoside Solution:  Add indoxyl-
β- D -glucoside to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Ferric Chloride Solution:
Composition per 10.0mL:
FeCl 3 ·6H 2 O.................................................................................0.45g
Preparation of Ferric Chloride Solution:  Add ferric chloride to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Phenolphthalein Solution:
Composition per 20.0mL:
Phenolphthalein biphosphate
tetrasodium salt.....................................................................0.15g
Preparation of Phenolphthalein Solution:  Add phenolphthalein
biphosphate tetrasodium salt to distilled/deionized water and bring vol-
ume to 20.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, phenolphthalein solution, ferric chloride solution,
and indoxyl-β- D -glucoside solution to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti-
cally add 8.0mL selective supplement solution, 20.0mL phenolphtha-
lein solution, 4.0mL ferric chloride solution, and 8.0mL indoxyl-β- D -
glucoside solution. Mix thoroughly. Pour into sterile Petri dishes or
aseptically distribute into sterile tubes.
Use: For the rapid isolation and presumptive identification of
Clostridium perfringens from food and water samples. A selective and
chromogenic medium for the presumptive identification of Clostridi-
um perfringens, especially from water samples. Recommended in Eu-
ropean Council Directive 98/83/EC for testing the quality of water in-
tended for human consumption. C. perfringens colonies have a charac-
teristic opaque yellow appearance. Most other Clostridium spp. will
appear as either purple colonies, due to the lack of sucrose fermenta-
tion, or blue/green colonies where the organism is still cleaving indox-
yl-β- D -glucoside and also fermenting sucrose.
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