Actinomycete Isolation Agar

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Actinomycete Isolation Agar
Composition per liter:
Agar ............................................................................................15.0g
Glycerol ........................................................................................5.0g
Sodium propionate........................................................................4.0g
Sodium caseinate ..........................................................................2.0g
K 2 HPO 4 .........................................................................................0.5g
Asparagine....................................................................................0.1g
MgSO 4 ·7H 2 O................................................................................0.1g
FeSO 4 ·7H 2 O...............................................................................1.0mg
pH 8.1± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components, except glycerol, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat and bring to boiling. Add 5.0g of glycerol. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the isolation and cultivation of aerobic Actinomyces from soil
and water.