Actinomycete Growth Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Actinomycete Growth Medium
Composition per liter:
Succinic acid...............................................................................1.18g
L- Glutamine ................................................................................0.29g
CaCl 2 ·2H 2 O ..................................................................................0.2g
KH 2 PO 4 .........................................................................................0.2g
MgSO 4 ·7H 2 O................................................................................0.2g
NaCl..............................................................................................0.1g
m-Inositol....................................................................................0.09g
Ferric EDTA .............................................................................0.037g
MnSO 4 ·H 2 O...............................................................................4.5mg
H 3 BO 3 ........................................................................................1.5mg
ZnSO 4 ·7H 2 O..............................................................................1.5mg
Nicotonic acid............................................................................0.5mg
Pyridoxine-HCl..........................................................................0.5mg
Thiamine-HCl............................................................................0.1mg
CuSO 4 ·5H 2 O............................................................................0.04mg
Na 2 MoO 4 ·2H 2 O .....................................................................0.025mg
pH 6.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of actinomycetes.