Medium 4 m 1

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium 4 m 1

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Peptone .........................................................................................3.0g
Pancreatic digest of casein............................................................3.0g
Yeast extract..................................................................................3.0g
Maltose..........................................................................................2.0g
Lactose..........................................................................................1.0g
Sodium dichromate solution..................................................100.0mL
pH 7.0 ± 0.2 at 25°C
Sodium Dichromate Solution:
Composition per 100.0mL:
Sodium dichromate.....................................................................0.05g
Preparation of Sodium Dichromate Solution: Add sodium di-
chromate to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Preparation of Medium: Add components, except sodium dichro-
mate solution, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH
to 7.2. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add sterile sodium dichromate solution. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Corynebacterium sepedoni-
cum.