Medium for Lactobacilli (ATCC Medium 980)

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Lactobacilli
(ATCC Medium 980)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
Peptone .......................................................................................12.5g
Glucose....................................................................................... 11.0g
Sodium acetate............................................................................10.0g
Yeast extract..................................................................................5.5g
KH 2 PO 4 .......................................................................................0.25g
K 2 HPO 4 .......................................................................................0.25g
MgSO 4 ..........................................................................................0.1g
MnSO 4 ·4H 2 O..............................................................................0.05g
FeSO 4 ·7H 2 O................................................................................0.05g
pH 6.8 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8. Au-
toclave for 10 min at 15 psi pressure–120°C. Aseptically distribute into
sterile tubes or flasks.
Use:  For the cultivation and maintenance of Pediococcus acidilactici
and Bacillus species.