Medium for Erythrobacter longus (DSMZ Medium 695)

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Erythrobacter longus
(DSMZ Medium 695)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Peptone..........................................................................................2.0g
Soytone .........................................................................................1.0g
Yeast extract..................................................................................1.0g
Proteose peptone No.3..................................................................1.0g
Ferric citrate solution.................................................................2.0mL
Artificial seawater..................................................................700.0mL
pH 7.5 ± 0.2 at 25°C
Artificial Seawater:
Composition per liter:
NaCl........................................................................................23.477g
MgCl 2 ·6H 2 O..............................................................................4.981g
Na 2 SO 4 ......................................................................................3.917g
CaCl 2 ..........................................................................................1.12g
KCl.........................................................................................664.0mg
NaHCO 3 .................................................................................192.0mg
H 3 BO 3 ......................................................................................26.0mg
SrCl 2 ........................................................................................24.0mg
KBr ............................................................................................6.0mg
NaF ............................................................................................3.0mg
Preparation of Artificial Seawater:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Ferric Citrate Solution:
Composition per 10.0mL:
Ferric citrate..................................................................................0.5g
Preparation of Ferric Citrate Solution : Add ferric citrate to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Add components, except artificial sea wa-
ter, to distilled/deionized water and bring volume to 300.0mL. Mix thor-
oughly. Adjust pH to 7.5. Aseptically add 700.0mL artificial sea water.
Mix thoroughly. Aseptically and anaerobically distribute into sterile
tubes or bottles.
Use:  For the cultivation of Erythrobacter longus.