Medium with EDTA as Carbon Source (DSMZ Medium 473)

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium with EDTA as Carbon Source
(DSMZ Medium 473)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
MgSO 4 ·7H 2 O..............................................................................0.49g
Na 2 -EDTA.....................................................................................0.2g
Ferric ammonium citrate.............................................................0.08g
Ca(NO 3 ) 2 ·4H 2 O...........................................................................0.02g
Phosphate solution...................................................................10.0mL
Trace elements solution SL-6 ....................................................5.0mL
Vitamin solution.........................................................................5.0mL
pH 7.5 ± 0.2 at 25°C
Phosphate Solution:
Composition per 10.0mL:
KH 2 PO 4 .....................................................................................0.272g
Preparation of Phosphate Solution:  Add KH 2 PO 4 to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Vitamin Solution:
Composition per 100.0mL:
KH 2 PO 4 .....................................................................................0.272g
Biotin ..........................................................................................0.08g
Folic acid.....................................................................................0.08g
Thiamin-HCl...............................................................................0.08g
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·H 2 O ............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· ·2H 2 O................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 3.4.
Preparation of Medium: Add components, except vitamin solution
and phosphate solution, to 985.0mL distilled/deionized water. Adjust
pH to 7.5. Autoclave for 15 min at 15 psi pressure–121°C. Pour into ster-
ile Petri dishes (20.0mL per Petri dish). Cool to room temperature.
Aseptically add 10.0mL sterile phosphate solution and 5.0mL sterile
vitamin solution. Mix thoroughly. Aseptically distribute to sterile tubes
or flasks.
Use: For the cultivation of unclassified bacterium DSM6780.