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Medium E for Sulfate Reducers (Postgate’s Medium E for Sulfate Reducers)
[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]
Medium E for Sulfate Reducers
(Postgate’s Medium E for Sulfate Reducers)
Sodium lactate ..............................................................................3.5g
MgCl 2 ·6H 2 O .................................................................................2.0g
NH 4 Cl ...........................................................................................1.0g
Na 2 SO 4 ..........................................................................................1.0g
CaCl 2 ·2H 2 O ..................................................................................1.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
Ascorbic acid................................................................................0.1g
Thioglycollic acid.........................................................................0.1g
FeSO 4 ·7H 2 O..............................................................................0.004g
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add components, except ascorbic acid
and thioglycollic acid, to tap water and bring volume to 1.0L. For ma-
rine bacteria, NaCl may be added or seawater used in place of tap wa-
ter. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.6.
Thioglycolate and ascorbate should be added immediately prior to ster-
ilization. Distribute into screw-capped tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C.
Use: For the cultivation and enumeration of Desulfovibrio species and
Desulfotomaculum species as black colonies in deep agar cultures.
Also used for the isolation of pure cultures of Desulfovibrio species
and Desulfotomaculum species.
(Postgate’s Medium E for Sulfate Reducers)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar............................................................................................15.0gSodium lactate ..............................................................................3.5g
MgCl 2 ·6H 2 O .................................................................................2.0g
NH 4 Cl ...........................................................................................1.0g
Na 2 SO 4 ..........................................................................................1.0g
CaCl 2 ·2H 2 O ..................................................................................1.0g
Yeast extract..................................................................................1.0g
KH 2 PO 4 .........................................................................................0.5g
Ascorbic acid................................................................................0.1g
Thioglycollic acid.........................................................................0.1g
FeSO 4 ·7H 2 O..............................................................................0.004g
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add components, except ascorbic acid
and thioglycollic acid, to tap water and bring volume to 1.0L. For ma-
rine bacteria, NaCl may be added or seawater used in place of tap wa-
ter. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.6.
Thioglycolate and ascorbate should be added immediately prior to ster-
ilization. Distribute into screw-capped tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C.
Use: For the cultivation and enumeration of Desulfovibrio species and
Desulfotomaculum species as black colonies in deep agar cultures.
Also used for the isolation of pure cultures of Desulfovibrio species
and Desulfotomaculum species.
