Medium E for Bacillus

[所属分类:培养基配方] [发布时间:2021-5-19] [发布人:网站管理员2] [阅读次数:] [返回]

Medium E for Bacillus

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................50.0g
K 2 HPO 4 .......................................................................................10.6g
Sucrose........................................................................................10.0g
KH 2 PO 4 .........................................................................................5.3g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
MgSO 4 ........................................................................................0.25g
Trace salts solution...................................................................10.0mL
Trace Salts Solution:
Composition per liter:
MnSO 4 ·H 2 O..................................................................................3.0g
Disodium EDTA ...........................................................................1.0g
FeSO 4 ·7H 2 O..................................................................................0.1g
CaCl 2 ·2H 2 O...................................................................................0.1g
CoCl 2 ·6H 2 O ..................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
CuSO 4 ·5H 2 O...............................................................................0.01g
AlK(SO 4 ) 2 ·12H 2 O.......................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O ..........................................................................0.01g
Preparation of Trace Salts Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Aseptically distribute into sterile tubes or
flasks.
Use:  For the cultivation and maintenance of Bacillus species.