Actinomyces Broth

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Actinomyces Broth
Composition per liter:
K 2 HPO 4 .......................................................................................13.0g
Heart muscle, solids from infusion.............................................10.0g
Peptic digest of animal tissue .....................................................10.0g
Glucose.........................................................................................5.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................5.0g
Pancreatic digest of casein............................................................4.0g
KH 2 PO 4 .........................................................................................2.0g
(NH 4 ) 2 SO 4 ....................................................................................1.0g
L- Cysteine·HCl·H 2 O......................................................................1.0g
Soluble starch................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.01g
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 10 min at 15 psi pressure–121°C.
Use: For the maintenance or cultivation of a variety of anaerobic bac-
teria including Actinomyces species, Eubacterium species, Fusobacte-
rium species, Propionibacterium species, and others.