Actinomyces Agar

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Actinomyces Agar
Composition per liter:
Agar ............................................................................................20.0g
K 2 HPO 4 .......................................................................................13.0g
Heart muscle, solids from infusion.............................................10.0g
Peptic digest of animal tissue......................................................10.0g
Glucose .........................................................................................5.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................5.0g
Pancreatic digest of casein............................................................4.0g
KH 2 PO 4 .........................................................................................2.0g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
L- Cysteine·HCl·H 2 O......................................................................1.0g
Soluble starch................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.01g
pH 6.9 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. If a semisolid medium is desired, add
7.0g of agar instead of 20.0g. Mix thoroughly. Gently heat and bring to
boiling. Distribute into tubes or flasks. Autoclave for 10 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the maintenance or cultivation of a variety of anaerobic bac-
teria, including Actinomyces species, Eubacterium species, Fusobacte-
rium species, Propionibacterium species, and others.