Actidione HiVeg Agar Base without Actidione ® with Antibiotics

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Actidione HiVeg Agar Base without Actidione ®
with Antibiotics
Composition per liter:
Glucose.......................................................................................50.0g
Agar............................................................................................15.0g
Plant hydrolysate ..........................................................................5.0g
Yeast extract..................................................................................4.0g
KH 2 PO 4 .......................................................................................0.55g
KCl............................................................................................0.425g
CaCl 2 ·2H 2 O ..............................................................................0.125g
MgSO 4 ·7H 2 O............................................................................0.125g
Bromocresol Green..................................................................22.0mg
MnSO 4 ·4H 2 O.............................................................................2.5mg
FeCl 3 ..........................................................................................2.5mg
Antibiotic solution...................................................................10.0mL
pH 5.5 ± 0.2 at 25°C
Antibiotic Solution:
Composition per 10.0mL:
Ampicillin or streptomycin......................................................20.0mg
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except antibiotic solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Aseptically add 10.0mL antibiotic solution. Pour into sterile Petri dish-
es or leave in tubes.
Use: For the selective isolation of dermatophytes.