Actidione HiVeg Agar Base with Actidione ®

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Actidione HiVeg Agar Base with Actidione ®
Composition per liter:
Glucose .......................................................................................50.0g
Agar ............................................................................................15.0g
Plant hydrolysate...........................................................................5.0g
Yeast extract..................................................................................4.0g
KH 2 PO 4 .......................................................................................0.55g
KCl............................................................................................0.425g
CaCl 2 ·2H 2 O...............................................................................0.125g
MgSO 4 ·7H 2 O............................................................................0.125g
Bromocresol Green..................................................................22.0mg
MnSO 4 ·4H 2 O.............................................................................2.5mg
FeCl 3 ..........................................................................................2.5mg
Cycloheximide solution...........................................................10.0mL
pH 5.5 ± 0.2 at 25°C
Source: This medium, without actidione (cycloheximide), is avail-
able as a premixed powder from HiMedia.
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide..........................................................................0.025g
Preparation of Cycloheximide Solution: Add cycloheximide to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except cycloheximide
solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptically add 10.0mL cycloheximide solution. Pour into ster-
ile Petri dishes or leave in tubes.
Use: For the enumeration and detection of bacteria in specimens con-
taining large numbers of yeasts and molds.