Acidiphilium Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidiphilium Medium
Composition per liter:
(NH 4 ) 2 SO 4 .....................................................................................2.0g
K 2 HPO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.5g
KCl................................................................................................0.1g
Glucose solution ......................................................................10.0mL
Yeast extract solution...............................................................10.0mL
pH 3.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
D- Glucose......................................................................................1.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract..................................................................................0.3g
Preparation of Yeast Extract Solution : Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except glucose solu-
tion and yeast extract solution, to distilled/deionized water and bring
volume to 1080.0mL. Mix thoroughly. Gently heat and bring to boil-
ing. Adjust pH to 3.0 using 1N H 2 SO 4 . Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature. Before inoculation, asep-
tically add glucose solution and yeast extract solution. Mix thoroughly.
Use:  For the cultivation and maintenance of Acidiphilium cryptum.