Malt Extract Agar

[所属分类:培养基配方] [发布时间:2021-5-13] [发布人:网站管理员2] [阅读次数:] [返回]

Malt Extract Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Malt extract.................................................................................30.0g
Agar ............................................................................................20.0g
Chlortetracycline solution........................................................10.0mL
pH 5.5 ± 0.2 at 25°C
Chlortetracycline Solution:
Composition per 10.0mL:
Chlortetracycline......................................................................0.04mg
Preparation of Chlortetracycline Solution: Add chlortetracy-
cline to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except chlortetracy-
cline solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile chlortetracycline solution. Mix thoroughly. Pour into sterile
Petri dishes in 20.0mL volumes.
Use:  For the cultivation of yeasts and filamentous fungi (molds) from
cosmetics.