Malt Extract Agar

[所属分类:培养基配方] [发布时间:2021-5-13] [发布人:网站管理员2] [阅读次数:] [返回]

Malt Extract Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Malt extract.................................................................................30.0g
Agar............................................................................................15.0g
Mycological peptone ....................................................................5.0g
pH 5.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring until boiling. Distribute into tubes or flasks. Autoclave for 10
min at 15 psi pressure–115°C. Do not overheat or agar will not harden.
If a lower pH (3.5) is desired, cool medium to 55°C and aseptically add
100.0mL of sterile lactic acid. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the detection, isolation, and enumeration of yeasts and
molds. The addition of lactic acid suppresses bacterial growth.