Maintenance of L Antigen in Neisseria

[所属分类:培养基配方] [发布时间:2021-5-12] [发布人:网站管理员2] [阅读次数:] [返回]

Maintenance of L Antigen in Neisseria

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone No. 3...............................................................20.0g
Agar ............................................................................................15.0g
Na 2 HPO 4 .......................................................................................5.0g
NaCl..............................................................................................5.0g
Glucose .........................................................................................0.5g
Rabbit blood, defibrinated .....................................................100.0mL
pH 7.4–7.6 at 25°C
Preparation of Medium: Add components, except rabbit blood, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Gently heat while stirring until boiling. Autoclave for 20 min at 15
psi pressure–121°C. Cool to 60°C. Aseptically add 100.0mL of sterile,
defibrinated rabbit blood. Maintain at 75°C while shaking for 30 min.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Neisseria gonorrhoeae.