Maintenance HiVeg Medium for B. subtilis ATCC 6633

[所属分类:培养基配方] [发布时间:2021-5-12] [发布人:网站管理员2] [阅读次数:] [返回]

Maintenance HiVeg Medium for B. subtilis ATCC
6633

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Plant peptone.................................................................................6.0g
Plant hydrolysate...........................................................................4.0g
Yeast extract..................................................................................3.0g
Plant extract ..................................................................................1.5g
Glucose .........................................................................................1.0g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring until boiling. Autoclave for 15 min at 15 psi pressure–121°C.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Bacillus subtilis.