M3 Agar

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

M3 Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1020.0mL:

Agar ............................................................................................18.0g
Na 2 HPO 4 ...................................................................................0.732g
KH 2 PO 4 .....................................................................................0.466g
NaCl............................................................................................0.29g
Sodium propionate........................................................................0.2g
MgSO 4 ·7H 2 O................................................................................0.1g
CaCO 3 .........................................................................................0.02g
KNO 3 ..........................................................................................0.01g
FeSO 4 ·7H 2 O...............................................................................0.2mg
ZnSO 4 ·7H 2 O............................................................................0.18mg
MnSO 4 ·4H 2 O...........................................................................0.02mg
Cycloheximide solution...........................................................10.0mL
Thiamine·HCl solution.............................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide............................................................................0.05g
Preparation of Cycloheximide Solution: Add cycloheximide to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Thiamine·HCl Solution:
Composition per 10.0mL:
Thiamine·HCl ............................................................................4.0mg
Preparation of Thiamine·HCl Solution: Add thiamine·HCl to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except cycloheximide
solution and thiamine·HCl solution, to distilled/deionized water and
bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 10.0mL of sterile cycloheximide solution and
10.0mL of thiamine·HCl solution. Mix thoroughly. Pour into sterile Pe-
tri dishes or distribute into sterile tubes.
Use: For the selective isolation and cultivation of Nocardia species
and Rhodococcus species.