M1A Medium

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

M1A Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Sorbitol........................................................................................23.3g
Peptone..........................................................................................6.0g
Sucrose..........................................................................................3.3g
Pancreatic digest of casein............................................................3.3g
Beef heart infusion........................................................................2.0g
Glucose .........................................................................................1.3g
Yeast extract..................................................................................1.0g
Fructose.........................................................................................0.3g
Phenol Red...............................................................................20.0mg
Schneider’s Drosophila medium...........................................533.0mL
Fetal calf serum, heat inactivated ..........................................167.0mL
Fresh yeast extract solution .....................................................33.0mL
Penicillin solution......................................................................8.0mL
Schneider’s Drosophila Medium:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.7g
NaCl..............................................................................................2.1g
Yeast extract..................................................................................2.0g
Trehalose.......................................................................................2.0g
D- Glucose......................................................................................2.0g
L- Glutamine ..................................................................................1.8g
L- Lysine·HCl.................................................................................1.7g
L- Proline........................................................................................1.7g
KCl................................................................................................1.6g
Na 2 HPO 4 ·7H 2 O.............................................................................1.3g
L- Glutamic acid.............................................................................0.8g
L- Methionine.................................................................................0.8g
CaCl 2 , anhydrous..........................................................................0.6g
KH 2 PO 4 .........................................................................................0.5g
β-Alanine......................................................................................0.5g
L- Tyrosine .....................................................................................0.5g
L- Arginine.....................................................................................0.4g
L- Aspartic acid..............................................................................0.4g
L- Histidine.....................................................................................0.4g
L- Threonine...................................................................................0.4g
NaHCO 3 ........................................................................................0.4g
Glycine..........................................................................................0.3g
L- Serine.........................................................................................0.3g
L- Valine.........................................................................................0.3g
L- Isoleucine...................................................................................0.2g
L- Leucine ......................................................................................0.2g
L- Phenylalanine.............................................................................0.2g
α-Ketoglutaric acid.......................................................................0.2g
Fumaric acid.................................................................................0.1g
Malic acid.....................................................................................0.1g
Succinic acid.................................................................................0.1g
L- Cystine.......................................................................................0.1g
L- Tryptophan.................................................................................0.1g
L- Cysteine ...................................................................................0.06g
Preparation of Schneider’s Drosophila Medium: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Filter sterilize.
Penicillin Solution:
Composition per 10.0mL:
Penicillin........................................................................... 2,500,000U
Preparation of Penicillin Solution: Add penicillin to distilled/de-
ionized water and bring volume to 10.0mL. Filter sterilize.
Fresh Yeast Extract Solution:
Composition per 100.0mL:
Baker’s yeast, live, pressed, starch-free......................................25.0g
Preparation of Fresh Yeast Extract Solution : Add the live Bak-
er’s yeast to 100.0mL of distilled/deionized water. Autoclave for 90
min at 15 psi pressure–121°C. Allow to stand. Remove supernatant so-
lution. Adjust pH to 6.6–6.8. Filter sterilize.
Preparation of Medium: Add components—except Schneider’s
Drosophila medium, fetal calf serum, fresh yeast extract solution, and
penicillin solution— to distilled/deionized water and bring volume to
260.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 533.0mL of sterile Schneider’s Drosophila medium, 167.0mL of
sterile fetal calf serum, 33.0mL of sterile fresh yeast extract solution,
and 8.0mL of sterile penicillin solution. Mix thoroughly. Pour into ster-
ile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Spiroplasma species that
cause corn stunt.