M1 Medium

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

M1 Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

L- Leucine.......................................................................................2.0g
L- Alanine.......................................................................................1.0g
L- Isoleucine...................................................................................1.0g
L- Phenylalanine.............................................................................1.0g
L- Proline........................................................................................1.0g
L- Tryptophane...............................................................................1.0g
L- Asparagine .................................................................................0.5g
L- Lysine.........................................................................................0.5g
L- Methionine.................................................................................0.5g
L- Tyrosine......................................................................................0.4g
L- Valine .........................................................................................0.2g
L- Serine .........................................................................................0.2g
MgSO 4 ·7H 2 O................................................................................0.2g
NaCl..............................................................................................0.2g
KH 2 PO 4 .......................................................................................0.14g
L- Arginine .....................................................................................0.1g
L- Cysteine......................................................................................0.1g
L- Glycine.......................................................................................0.1g
L- Histidine.....................................................................................0.1g
L- Threonine...................................................................................0.1g
CaCl 2 ..........................................................................................2.0mg
FeCl 3 ·6H 2 O................................................................................2.0mg
Tris(hydroxymethyl)aminomethane
buffer (0.01M solution, pH 7.6).............................................1.0L
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add solid components to 1.0L of Tris
buffer. Mix thoroughly. Filter sterilize. Aseptically distribute into tubes
or flasks.
Use: For the cultivation of Myxococcus xanthus.