M Medium

[所属分类:培养基配方] [发布时间:2021-5-10] [发布人:网站管理员2] [阅读次数:] [返回]

M Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef...............................................................................................5.0g
Neopeptone...................................................................................4.0g
NaCl..............................................................................................1.6g
Glucose.........................................................................................0.5g
CaCl 2 ...........................................................................................0.06g
KH 2 PO 4 .......................................................................................0.06g
KCl..............................................................................................0.04g
Rabbit blood solution.............................................................200.0mL
Rabbit Blood Solution:
Composition per liter:
Rabbit blood...........................................................................500.0mL
Preparation of Rabbit Blood Solution: Add 500.0mL of whole
rabbit blood to 500.0mL of sterile distilled/deionized water. Freeze and
thaw twice to lyse blood cells.
Preparation of Medium: Trim beef to remove fat. Add 5.0g of lean
beef to 200.0mL of distilled/deionized water. Gently heat and bring to
boiling. Boil for 2–3 min. Filter through Whatman #2 filter paper. Add
other components, except rabbit blood solution. Bring volume to
800.0mL with distilled/deionized water. Mix thoroughly. Adjust pH to
7.2 with 1N NaOH. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 50°–55°C. Aseptically add 200.0mL of lysed rabbit blood so-
lution. Mix thoroughly. Aseptically distribute into sterile tubes or

flasks.

Use: For the cultivation of Herpetomonas megaseliae.