Acidianus brierleyi Medium

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidianus brierleyi Medium
Composition per liter:
Sulfur flowers .............................................................................10.0g
(NH 4 ) 2 SO 4 .....................................................................................3.0g
K 2 HPO 4 ·3H 2 O...............................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.5g
KCl............................................................................................... 0.1g
Ca(NO 3 ) 2 .....................................................................................0.01g
Yeast extract solution...............................................................10.0mL
pH 1.5–2.5 at 25°C
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract..................................................................................0.2g
Preparation of Yeast Extract Solution : Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except sulfur flowers
and yeast extract solution, to distilled/deionized water and bring volume
to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Sulfur flowers are sterilized sepa-
rately by steaming for 3 hr on 3 consecutive days. Aseptically combine
the basal solution, sterile sulfur flowers, and sterile yeast extract solution.
Adjust pH to 1.5–2.5 with 6N H 2 SO 4 .
Use: For the cultivation and maintenance of Acidianus brierleyi.