Acidaminococcus Medium VR

[所属分类:培养基配方] [发布时间:2019-9-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acidaminococcus Medium VR
Composition per liter:
Acid-hydrolyzed casein
(vitamin and salt free) ..........................................................20.0g
Glucose.........................................................................................5.0g
L- Cysteine·HCl·H 2 O....................................................................0.35g
DL- Tryptophan...............................................................................0.1g
Guanine.......................................................................................0.01g
Uracil ..........................................................................................0.01g
Hypoxanthine..............................................................................0.01g
Pyridoxal....................................................................................1.0mg
Calcium pantothenate ................................................................1.0mg
Thiamine...................................................................................50.0μg
Niacin........................................................................................50.0μg
Riboflavin.................................................................................50.0μg
p-Aminobenzoic acid................................................................10.0μg
Biotin..........................................................................................2.0μg
Folic acid ....................................................................................1.0μg
Vitamin B 12 .................................................................................1.0μg
VR salts A................................................................................30.0mL
VR salts B..................................................................................4.0mL
pH 7.0 ± 0.2 at 25°C
VR Salts A:
Composition per 500.0mL:
Na 2 HPO 4 .....................................................................................37.5g
KH 2 PO 4 .......................................................................................12.5g
Preparation of VR Salts A: Add components to distilled/deionized
water and bring volume to 500.0mL. Mix thoroughly.
VR Salts B:
Composition per liter:
MgSO 4 ·7H 2 O..............................................................................24.0g
CaCl 2 ·2H 2 O ..................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.5g
ZnSO 4 ·7H 2 O...............................................................................0.25g
MnSO 4 ·H 2 O................................................................................0.25g
CoCl 2 ·6H 2 O................................................................................0.25g
VSO 4 ·7H 2 O.................................................................................0.25g
Na 2 MoO 4 ·2H 2 O..........................................................................0.25g
CuSO 4 ·5H 2 O.............................................................................0.125g
Preparation of VR Salts B: Add components to distilled/deionized
water and bring volume to 700.0mL. Add 2.0mL of concentrated HCl and
heat until dissolved. Add 5.0g of nitrilotriacetic acid to 300.0mL distilled/
deionized water. Adjust pH with 10N NaOH to 7.0. Stir vigorously and
slowly add the nitrilotriacetic acid solution to the larger volume of salt so-
lution until dissolved. Add distilled/deionized water and bring volume to
1.0L. Filter through paper. Store in a cool place.
Preparation of Medium: Filter sterilize vitamins as separate solu-
tion. Add aseptically to sterile basal medium. If necessary, adjust pH
with solid K 2 CO 3 to 7.0. Prepare and distribute medium anaerobically
using Hungate techniques with 100% N 2 gas.
Use: For the cultivation and maintenance of Acidaminococcus fermen-
tans.