LPM Agar with Esculin and Ferric Iron

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

LPM Agar with Esculin and Ferric Iron

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
Glycine anhydride.......................................................................10.0g
LiCl...............................................................................................5.0g
NaCl..............................................................................................5.0g
Pancreatic digest of casein............................................................5.0g
Peptic digest of animal tissue .......................................................5.0g
Beef extract...................................................................................3.0g
Phenylethyl alcohol ......................................................................2.5g
Esculin..........................................................................................1.0g
Ferric ammonium citrate...............................................................0.5g
Moxalactam solution .................................................................2.0mL
pH 7.3 ± 0.2 at 25°C
Moxalactam Solution:
Composition per 10.0mL:
Moxalactam ..................................................................................0.1g
Preparation of Moxalactam Solution: Add moxalactam to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except moxalactam so-
lution, to distilled/deionized water and bring volume to 998.0mL. Mix
thoroughly. Gently heat while stirring and bring to boiling. Autoclave
for 12 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 2.0mL of sterile moxalactam solution. Mix thoroughly. Pour into
sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Listeria monocytogenes.