LPM Agar (Lithium Phenylethanol Moxalactam Agar)

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

LPM Agar
(Lithium Phenylethanol Moxalactam Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Glycine anhydride.......................................................................10.0g
Casein enzymic hydrolysate .........................................................5.0g
Peptic digest of animal tissue........................................................5.0g
Beef extract...................................................................................3.0g
LiCl...............................................................................................5.0g
NaCl..............................................................................................5.0g
Phenylethyl alcohol.......................................................................2.5g
Selective supplement solution .................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Moxalactam .............................................................................20.0mg
Preparation of Selective Supplement Solution:  Add moxalac-
tam to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Caution: Lithium chloride is harmful. Avoid bodily contact and inha-
lation of vapors. On contact with skin, wash with plenty of water im-
mediately.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add selective supplement solution.
Mix thoroughly. Pour into Petri dishes or aseptically distribute into
sterile tubes.
Use: For the isolation and cultivation of Listeria monocytogenes from
food and dairy products.