Lowenstein-Jensen Medium with Streptomycin

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Lowenstein-Jensen Medium with Streptomycin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

dona species, Nocardia species, Rhodococcus species, and Tsuka-
murella paurometabolum.
Composition per 1610.0mL:

Potato starch................................................................................30.0g
Asparagine....................................................................................3.6g
KH 2 PO 4 .........................................................................................2.4g
Magnesium citrate.........................................................................0.6g
Malachite Green............................................................................0.4g
MgSO 4 ·7H 2 O..............................................................................0.24g
Homogenized whole egg ............................................................. 1.0L
Glycerol ...................................................................................12.0mL
Streptomycin solution..............................................................10.0mL
Homogenized Whole Egg:
Composition per liter:
Whole eggs ................................................................................18–24
Preparation of Homogenized Whole Egg:  Use fresh eggs, less
than 1 week old. Scrub the shells with soap. Let stand in a soap solution
for 30 min. Rinse in running water. Soak eggs in 70% ethanol for 15
min. Break the eggs into a sterile container. Homogenize by shaking.
Filter through four layers of sterile cheesecloth into a sterile graduated
cylinder. Measure out 1.0L.
Streptomycin Solution:
Composition per 10.0mL:
Streptomycin..............................................................................0.1mg
Preparation of Streptomycin Solution: Add streptomycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add glycerol to 600.0mL of distilled/de-
ionized water. Mix thoroughly. Add remaining components, except
fresh egg mixture and streptomycin solution. Mix thoroughly. Gently
heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add 1.0L of homogenized
whole egg and 10.0mL of sterile streptomycin solution. Mix thorough-
ly. Distribute into sterile screw-capped tubes. Place tubes in a slanted
position. Inspissate at 85°C (moist heat) for 45 min.
Use: For the cultivation and differentiation of Mycobacterium species.
Mycobacterium tuberculosis appears as granular, rough, dry colonies.
Mycobacterium kansasii appears as smooth to rough photochromoge-
nic colonies. Mycobacterium gordonae appears as smooth yellow-
orange colonies. Mycobacterium avium appears as smooth, colorless
colonies. Mycobacterium smegmatis appears as wrinkled, creamy

white colonies. Also used for the cultivation and maintenance of Gor-

dona species, Nocardia species, Rhodococcus species, and Tsuka-
murella paurometabolum.