Lowenstein-Jensen Medium with Sodium Chloride

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Lowenstein-Jensen Medium with Sodium Chloride

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1600.0mL:

NaCl............................................................................................80.0g
Potato starch................................................................................30.0g
Asparagine....................................................................................3.6g
KH 2 PO 4 .........................................................................................2.4g
Magnesium citrate ........................................................................0.6g
Malachite Green............................................................................0.4g
MgSO 4 ·7H 2 O..............................................................................0.24g
Homogenized whole egg ............................................................. 1.0L
Glycerol ...................................................................................12.0mL
Homogenized Whole Egg:
Composition per liter:
Whole eggs................................................................................18–24
Preparation of Homogenized Whole Egg:  Use fresh eggs, less
than 1 week old. Scrub the shells with soap. Let stand in a soap solution
for 30 min. Rinse in running water. Soak eggs in 70% ethanol for 15
min. Break the eggs into a sterile container. Homogenize by shaking.
Filter through four layers of sterile cheesecloth into a sterile graduated
cylinder. Measure out 1.0L.
Preparation of Medium: Add glycerol to 600.0mL of distilled/de-
ionized water. Mix thoroughly. Add remaining components, except fresh
egg mixture. Mix thoroughly. Gently heat while stirring and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C.
Aseptically add 1.0L of homogenized whole egg. Mix thoroughly. Dis-
tribute into sterile screw-capped tubes. Place tubes in a slanted position.
Inspissate at 85°C (moist heat) for 45 min.
Use: For the cultivation of Mycobacterium smegmatis and other salt-
tolerant Mycobacterium species.