Lombard-Dowell Broth (LD Broth)

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Lombard-Dowell Broth
(LD Broth)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................2.5g
Agar..............................................................................................0.7g
L- Tryptophan.................................................................................0.2g
Na 2 SO 3 ..........................................................................................0.1g
NaOH (1N).................................................................................5.0mL
Hemin solution...........................................................................1.0mL
Vitamin K 1 solution...................................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Hemin Solution:
Composition per 100.0mL:
Hemin ...........................................................................................1.0g
NaOH (1N solution).................................................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-

tilled/deionized water.

Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................................................1.0g
Ethanol.....................................................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly.
Preparation of Medium: Add tryptophan to 5.0mL of NaOH. Mix
thoroughly. Add remaining components. Bring volume to 1.0L with dis-
tilled/deionized water. Mix thoroughly. Gently heat and bring to boiling.
Adjust pH to 7.5. Distribute into screw-capped tubes in 7.0mL volumes.
Autoclave for 15 min at 15 psi pressure–121°C. Cool tubes, with caps
loose, under 85% N 2 + 10% H 2 + 5% CO 2 . Tighten caps.
Use: For the cultivation of a wide variety of anaerobic bacteria.