Lombard-Dowell Bile Agar (LD Bile Agar)

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Lombard-Dowell Bile Agar
(LD Bile Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................20.0g
Oxgall .........................................................................................20.0g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................2.5g
D- Glucose......................................................................................1.0g
L- Cystine.......................................................................................0.4g
L- Tryptophan.................................................................................0.2g
Na 2 SO 3 ..........................................................................................0.1g
Hemin ......................................................................................10.0mg
Bile.........................................................................................200.0mL
NaOH (1N).................................................................................5.0mL
Vitamin K 1 solution...................................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................................................1.0g
Ethanol.....................................................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly.
Preparation of Medium: Add hemin and  L -cystine to 5.0mL of
NaOH. Mix thoroughly. Add remaining components. Bring volume to
1.0L with distilled/deionized water. Mix thoroughly. Gently heat and
bring to boiling. Distribute into tubes or flasks. Pour into sterile Petri
dishes.
Use: For the cultivation and identification of a variety of obligate anaero-
bic bacteria in the presence of 20% bile.