Loeffler HiVeg Medium Base with Asolectin

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Loeffler HiVeg Medium Base with Asolectin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.........................................................................................2.5g
Plant extract..................................................................................2.5g
Plant special peptone ....................................................................2.5g
NaCl............................................................................................1.25g
Asolectin solution....................................................................10.0mL
pH 7.6 ± 0.2 at 25°C
Source: This medium, without asolectin, is available as a premixed
powder from HiMedia.
Asolectin Solution:
Composition per 100.0mL:
Asolectin.......................................................................................1.0g
Ethanol...................................................................................100.0mL
Preparation of Asolectin Solution: Add 1.0g of asolectin to eth-
anol and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Distribute into screw-
capped tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Tetrahymena patula.