Loeffler Blood Serum Medium

[所属分类:培养基配方] [发布时间:2021-5-8] [发布人:网站管理员2] [阅读次数:] [返回]

Loeffler Blood Serum Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Beef blood serum...................................................................750.0mL
Dextrose broth........................................................................250.0mL

pH 7.1 ± 0.2 at 25°C

Dextrose Broth:
Composition per liter:
Enzymatic digest of protein..........................................................2.5g
Glucose.......................................................................................1.25g
NaCl............................................................................................1.25g
Beef extract.................................................................................0.75g
Preparation of Dextrose Broth: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Combine 750.0mL of beef blood serum
with 250.0mL of dextrose broth. Mix thoroughly. Distribute into
screw-capped tubes. Slant tubes in the autoclave. Close the autoclave
door loosely. Autoclave for 10 min at 0 psi pressure–100°C. Close the
autoclave door tightly. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Corynebacterium diphtheriae. For demon-
stration of pigment production and proteolysis by Corynebacterium
diphtheriae.