LMX Broth Modified, Fluorocult (Fluorocult LMX Broth, Modified)

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

LMX Broth Modified, Fluorocult
(Fluorocult LMX Broth, Modified)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose..........................................................................................5.0
NaCl................................................................................................5.0
Sorbitol ...........................................................................................1.0
Tryptophan......................................................................................1.0
K 2 HPO 4 .........................................................................................2.7g
KH 2 PO 4 .........................................................................................2.0g
Lauryl sulfate sodium salt.............................................................0.1g
1-Isopropyl-β- D -1-thio-galactopyranoside (IPTG) ......................0.1g
5-Bromo-4-chloro-3-indolyl-β- D -galactopyranoside (X-GAL).0.08g
4-Methylumbelliferyl-β- D -glucuronide......................................0.05g
pH: 6.8 ± 0.2 at 25°C
Source: This medium is available from Merck.
Preparation of Medium:  Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Distribute into test tubes.
Autoclave for 15 min at 15 psi pressure–121°C. The broth is clear and
yellowish brown.
Use: For the simultaneous detection of total coliforms and E. coli in
water, food, and diary products by the fluorogenic procedure. A color
change of the broth from yellow to blue-green indicates the presence of
coliforms. A blue fluorescence under long-wave UV light permits the
rapid detection of E.coli. As tryptophan is added to the broth, the indole
reaction is easily done by adding KOVACS reagent. The formation of
a red ring additionally confirms the presence of E. coli.