Liver Veal Egg Yolk Agar

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Liver Veal Egg Yolk Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1080.0mL:

Liver veal agar..............................................................................1.0L
Egg yolk emulsion, 50%..........................................................80.0mL
pH 7.3 ± 0.2 at 25°C
Liver Veal Agar:
Composition per liter:
Veal, infusion from ...................................................................500.0g
Beef liver, infusion from.............................................................50.0g
Gelatin.........................................................................................20.0g
Proteose peptone.........................................................................20.0g
Agar ............................................................................................15.0g
Soluble starch..............................................................................10.0g
Glucose .........................................................................................5.0g
NaCl..............................................................................................5.0g
Casein............................................................................................2.0g
NaNO 3 ...........................................................................................2.0g
Enzymatic digest of protein..........................................................1.3g
Pancreatic digest of casein............................................................1.3g
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Liver Veal Agar: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Gently heat
and bring to boiling. Distribute into tubes or flasks. Make sure that
some liver and veal particles are transferred to each tube. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 50°C.
Egg Yolk Emulsion, 50%:
Composition per 100.0mL:
Chicken egg yolks............................................................................ 11
Whole chicken egg ............................................................................1
NaCl (0.9% solution)...............................................................50.0mL
Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 1 chicken egg.
Beat to form emulsion. Measure 50.0mL of egg yolk emulsion and add
to 50.0mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize.
Warm to 45°–50°C.
Preparation of Medium: To 1.0L of cooled sterile liver veal agar,
aseptically add 80.0mL of sterile egg yolk emulsion, 50%. Mix thor-
oughly. Pour into sterile Petri dishes. Dry plates at 35°C for 24 hr.
Use: For the cultivation of a variety of anaerobic organisms.