Litmus Milk

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Litmus Milk

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Skim milk..................................................................................100.0g
Azolitmin......................................................................................0.5g
Na 2 SO 3 ..........................................................................................0.5g
pH 6.5 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and Oxoid Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 20 min at 10
psi pressure–115°C.
Use: For the maintenance of lactic acid bacteria and for the differentia-
tion of several bacteria, especially Clostridium species, based on their
action on milk. Bacteria that do not ferment carbohydrates, such as Pro-
teus vulgaris or Moraxella lacunata, show no change in the azolitmin lit-
mus indicator. Bacteria that ferment lactose or glucose with the produc-
tion of gas, such as Clostridium perfringens, turn the medium pink and
frothy. Bacteria that proteolytically degrade milk lactalbumin turn the
medium blue. Bacteria that coagulate milk casein form a curd or clot.
Bacteria that peptonize casein, such as Pseudomonas aeruginosa, show
a dissolution of the clot.