Litmus Lactose HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Litmus Lactose HiVeg Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant peptone ..............................................................................23.0g
Lactose........................................................................................20.0g
Agar............................................................................................15.0g
Plant extract..................................................................................5.0g
NaCl..............................................................................................5.0g
Synthetic detergent No. V.............................................................2.0g
Litmus...........................................................................................0.5g
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMe-
dia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the maintenance of lactic acid bacteria and differentiation of
bacteria on the basis of lactose fermentation. Bacteria that ferment lac-
tose appear as red colonies and others as dark blue-purple colonies.