Listeria Identification HiVeg Agar Base with PALCAM Selective Supplement (PALCAM)

[所属分类:培养基配方] [发布时间:2021-5-7] [发布人:网站管理员2] [阅读次数:] [返回]

Listeria Identification HiVeg Agar Base
with PALCAM Selective Supplement
(PALCAM)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant peptone ..............................................................................23.0g
LiCl.............................................................................................15.0g
Agar............................................................................................13.0g
Mannitol......................................................................................10.0g
NaCl..............................................................................................5.0g
Starch............................................................................................1.0g
Esculin..........................................................................................0.8g
Ammonium ferric citrate ..............................................................0.5g
Glucose.........................................................................................0.5g
Phenol Red..................................................................................0.08g
PALCAM selective supplement...............................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without PALCAM selective supplement, is
available as a premixed powder from HiMedia.
Caution:  LiCl is harmful. Avoid bodily contact and inhalation of va-
pors. On contact with skin wash with plenty of water immediately.
PALCAM Selective Supplement:
Composition per 10.0mL:
Ceftazidime..............................................................................20.0mg
Polymyxin B............................................................................10.0mg
Acriflavine·HCl .........................................................................5.0mg
Preparation of PALCAM Selective Supplement: Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix

thoroughly. Filter sterilize.

Preparation of Medium: Add components, except PALCAM se-
lective supplement, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile PALCAM selective supplement. Mix thoroughly. Pour into
sterile Petri dishes.
Use: For the selective isolation and identification of Listeria monocy-
togenes and other Listeria species from foods.